How to Use the Cotinine Saliva Test

Before You Begin

• Do not eat, drink, or use e-cigarettes/vapes for at least 10 minutes before starting.

• Wash your hands and make sure you’re in a clean, flat space.

Step-by-Step

1. Open the test kit – Remove the test device and collection pad.

2. Collect your sample – Place the pad in your mouth until it is soaked with saliva (usually 2–3 minutes).

3. Insert the pad – Place the pad into the test device as shown in the instructions.

4. Wait – Put the device on a flat surface and wait the recommended time (usually 5 minutes).

5. Read the results – Look at the test window:

   • Two lines = Negative (no cotinine detected)

   • One line = Positive (cotinine detected)

   • No line = Invalid (test didn’t work, repeat if directed)

6. Take a photo – Snap a clear picture of the results.

7. Upload your photo – Go to the Insight app and submit the photo through the upload option.

Tips for Success

• Good lighting helps you capture a clear photo.

• If the test seems unclear or fails, contact the study team for support.

• Keep the device flat and undisturbed during the wait time.